Jun 27, 1970 · At this point, the reverse transcriptase enzyme is added, and this enzyme proceeds to utilize the mRNA strand as a template for the synthesis of a complementary DNA strand.

Jul 15, 2023 · For all samples, negative controls without reverse transcriptase enzyme were also prepared. Quantitative real-time PCR (qPCR) for satellite DNA expression analysis

Nov 4, 2025 · Reverse transcription polymerase chain reaction (RTPCR) is a technique to detect or quantify RNA. It uses a reverse transcriptase enzyme to convert RNA to DNA, followed by PCR to …

Jul 25, 2022 · Here we seek to achieve reverse-transcriptase quantitative PCR (RT-qPCR) in a single reaction vessel containing PCR chemistry, fluorescent probes and plasmonic nanoparticles, with the …

Jul 18, 2025 · The combination of PCR with reverse transcription (RT) laid the basis for various powerful methods in the research field of molecular biotechnology and clinical diagnostics 1, 2. RT-PCR is …

The qualitative and/or quantitative analysis of transcripts by PCR amplification is a method of choice in both molecular biology research and medical diagnostics1. It is widely used to detect and ...

Feb 18, 2022 · On the other hand, the reverse transcription polymerase chain reaction (RT-PCR) is the most common and accepted as the gold standard method for the viral detection 9.

Oct 9, 2003 · Standardization and quality control studies of ‘real-time’ quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia ...

Oct 14, 2023 · Genome sequencing and real-time reverse transcriptase PCR (rRT-PCR) assays that target single nucleotide polymorphisms (SNPs), deletions, or insertions have been used extensively …

Feb 17, 2025 · For the first round of PCR (PCR 1), the amplification primers containing Illumina forward and reverse adapters (listed in Supplementary Table 5) were used to amplify the genomic sites of …