Gelatin Zymography Tutorial 5: Quantifying MMP Activity with Image J and Normalizing Samples
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Gelatin Zymography Tutorial 5: Quantifying MMP Activity with Image J and Normalizing Samples
Here we demonstrate how to quantify bands of MMP activity in a Gelatin Zymogram using Image J, and then normalize the MMP activity of samples to an MMP-2 standard in Excel. We also explain the rationale behind normalizing samples with an MMP standard, which is to account for the variations which normally occur from gel to gel. Links mentioned ...
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