Reverse transcriptase (RT)-PCR: Principles, Applications | Microbe Online
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Arrange the steps of diagnosing AIDS using the RT PCR method in the right order.I. DNA, engineered primers specific to HIV genes, and nucleotides along with buffers are incubated in a thermal cycler.II. Viral RNA is extracted from the patient’s plasma or blood sample.III. The PCR product is run on a gel to separate and identify the PCR amplified viral genes.IV. Reverse transcriptase is used to convert the RNA to cDNA.
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